Centre for Excellence In Biotechnology


Biotechnology is one of the fastest growing multi-disciplinary branches of science. The past couple of decades have witnessed a “Biotechnology Boom” worldwide in the form of development of a wide range of new products including antibiotics, recombinant and synthetics vaccines, monoclonal antibodies, microbial proteins, recombinant hormones and development of valuable transgenic and cloned animals. Genetic engineering and Human Genome project, propelled by Bioinformatics has opened new vistas of Genomics, Proteomics & Pharmaco-genomics research for healthcare, involving Gene Therapy and Stem Cell research. Looking at the tremendous importance of biotechnology for sustainable use and its wide application in biosciences, medical sciences and other fields, a Centre of Excellence in Biotechnology (CEBT) has been established at the MPCST in 2008. The laboratory is well equipped and is also ISO (9001:2008) certified. 



Objective


·   Human resource development programs for the teachers, students and the researchers in universities and other S&T institutions for their skill up-gradation.

·         To develop and promote R&D projects for application of biotechnology for ensuring sustainable use of natural resources of the state.

·  To work for developing appropriate linkages and policies for promoting application of biotechnology in various sectors to ensure value addition and sustainable development of natural resources in the state.

·         Undertake R&D in various branches of biotechnology and its applications in different areas.

·         To Explore the natural resources of Madhya Pradesh, their conservation and management for the benefit of human health and society with the application of biotechnology,  and also for the future prospects, 

·   To promote Research and Development on all aspects of molecular biology, leading to improvements in agriculture, healthcare and the environment and also with development of molecular markers for authentication of plants, animals and microbes and its quality control with the limited facilities available in the Madhya Pradesh.

 

Major Thrust areas for R&D at CEBT

Ø       Agro-biotechnology including animal husbandry and veterinary science.

Ø    Biotechnology for processing and value addition of agro & forestry based products and other natural resources of M.P.

Ø       Biotechnology interventions in organic cultivation based products.

Ø       Biotechnology for diagnostics, pharma, healthcare and drinking water.

Ø       Bio-energy and biotechnology based industries. 

Ø       Biotechnology for environment management.

R&D & Academic Activities

Ø       Dissertation & project work to M.Sc., M.Phil. students.

Ø       Facility for scientists/facilities for 2-6 months.

Ø       Refresher courses for University/College teachers for 21-25 days.

Ø       Training programs on molecular biology, microbiology and HPTLC for 3-7 days.

 Ø        Analysis facility to the researchers of the M.P. working in the area of biotechnology. 



Facilities for R & D


Major Thrust areas for R&D at CEBT

  • Agro-biotechnology including animal husbandry and veterinary science.
  • Biotechnology for processing and value addition of agro & forestry based products and other natural resources of M.P.
  • Biotechnology interventions in organic cultivation based products.
  • Biotechnology for diagnostics, pharma, healthcare and drinking water.
  • Bio-energy and biotechnology based industries. 
  • Biotechnology for environment management.

 

R&D & Academic Programmes

  • Dissertation & project work to B. Tech., M. Tech. M.Sc., M.Phil. and Ph.D. students.
  • Facilities for scientists may pursue their research work (2-6 months) at the Centre.
  • Refresher courses for universities/college teachers 15 to 21 days.
  • Training programs on molecular biology, microbiology and HPTLC for 3-7 days.
  • Analyses facilities to the researchers working in the area of biotechnology/applied research. 

 

Testing Facility & Their Rates

  • DNA Sequencing Rs. 400/- per samples for R&D samples.
  •  Bacterial load in water samples @ Rs. 100 /- per sample.
  • Soil, herbal drugs & cosmetic samples (bacterial & fungal load) @ Rs. 175 /- per sample.
  • Antimicrobial testing @ Rs. 35/- per sample/ per organism.
  •  Photo microscopy @ Rs. 30 /- per slide.
  • Microbial fermentation study @ Rs. 4000/- per strain/enzyme or product.
  • Lyophilization of microbial cultures Rs. 150/- per vial preparation.
  • RAPD-PCR molecular work (plants, bacterial, fungal and animals) Rs. 4000/- per seven samples with 4 RAPD primers.
  • Karyotyping analysis (Rs. 1,000/-per species, photography extra @Rs. 30/- snap).
  • HPTLC analysis of commercial samples (@Rs. 4500/- for 10 samples) & agriculture farmers/R&D samples (Rs. 2500/- for 10 samples).Laboratory Facilities
  •  

Major Equipments available in Biotechnology Laboratory

v  Gel Documentation System (Alpha Innotech, USA), DNA Sequencer (3500 Genetic Analyzer, Applied Biosystems, USA), HPTLC (Linomet-V, ADC-II, Scanner-III, CAMAG, Switzerland), Fluorescence Microscope (Zesis, Carl Zeiss, Germany), Thermal Cycler PCR (ep-Gradient-S, Eppendorf, Germany & Corbett research, Australia), Deep Freezer -86 0C (U 410-86,New Brunswick, USA), CO2 incubator (Galaxy B+ 170-400P,Scientech, India), Bacteriological Incubator (BD154,Labotech, India), Laminar Air Flow (Elie, Elite, India), Binocular Microscope (Galen, Leica Galen III, Germany), Inverted Microscope (CH2, Olympus CH-2, India), Phase Contrast microscope (MLXi, Magnus MLXi, India), Lyophilizer (Lyodel, Delvac, India), UV-Vis Spectrophotometer (SL-159, Elico/SL159, India), ELISA (Sunrise Basic Tecan, Tecan, Switzerland), Centrifuge (J2-21, Beckman /J2-21,U.S.A), Dry Bath (SLM-DB-120, Genei, India), pH meter (780 metrom, Metrohm, Germany), Centrifuge (MPW-351R, MPW, Poland), Refrigerated Incubator Shaker (Innova-4230, Innova-4230,USA), Laminar Flow Bench (SE113,Scientech, India), Microtome Automatic Yorco (YSI-118, York, India), Milli-Q Water Purification (Synthesis-8, Millipore, India). 

 



Future Plans


 

 

  • Whole genome sequencing/next generation sequencing of selected animals of the Madhya Pradesh.
  • Establishment of gene bank for endangered species of Madhya Pradesh conservation purposes special reference to conservation priorities.
  • Pharmacological studies of selected medicinal plants and their toxicity studies.
  • Conservation genetics and molecular identification of micro-organisms, plants and animals using various molecular tools.
  • Genetic diversity, DNA fingerprinting, genetic profiling, DNA sequencing, etc.
  • Rapid propagation of high yielding plant by plants tissue culture and their molecular profiling.
  • Preparation of microbial consortium for important microbes of the Madhya Pradesh from hot springs etc. 
  • Development of DNA Barcode for identification and authentication of plant species, especially economically important medicinal plants of Madhya Pradesh. 


Projects -Current-Ongoing-Completed


 Current Projects (Internal Assignments)

v    Development of DNA barcoding and SCAR markers for important animal species of India using mtcox1 gene for authentication and identification of ambiguous samples.

v    Molecular phylogeny and characterization of selected animals species of Madhya Pradesh using variable and conserved gene profiling.

v         Healthcare studies of tribal populations of Madhya Pradesh using advance molecular approaches.

v      Preparation of microbial consortium and molecular studies of important microbes with reference to gene profiling.

v     Studies on genetic diversity and conservation of germplasm of economically important medicinal plants of Madhya Pradesh & Preparation of database of medicinal plants species.

v    HPTLC based characterization of selected medicinal plants of Madhya Pradesh and estimation of their medicinal properties.

 

Ongoing Projects (External Funded Projects)

1.       Title: Development of SCAR-DNA Markers for Identification and Authentication of Mahseer Tor (declared as State Fish of Madhya Pradesh) and their Phylogenetic Position through MTCOX1 gene”. Funding Agency: DST-SERB, GOI, New Delhi. Sanctioned Amount: 10.80 Lakhs. Duration: Two Years (2015-2017).  PI: Dr. R.K. Garg, Joint Project Director, MPCOST, Bhopal.

2.     Title: “Mapping of High Fluoride Concentration Areas and Prevalence of Dental Fluorosis among Primary and Secondary Schools Children’s of District Sheopur (A scheduled tribe district), Madhya Pradesh”. Funding Agency: DST-NRMS Division, GOI, New Delhi. Sanctioned Amount: 20.00 Lakhs. Duration: Two Years (2015-17). PI: Dr. R.K. Garg, Joint Project Director, MPCOST, Bhopal.

 

3.     Title: “Development of DNA barcode database for identification and diversity assessment of economically important medicinal plants of Madhya Pradesh”. Funding Agency: DST-SERB, New Delhi. Sanction Amount: 5.40 Lakhs. Durations: One Year (2015-2016). PI: Dr. Pramod Kumar Sairkar

Completed Projects (Outside Sanctioned Projects)

Title: Next Generation Sequencing (NGS): A Discussion Forum towards Whole Genome Sequencing and Library Preparation (3 days workshop). Funding Agency: DST-SERB, GOI, New Delhi. Sanctioned Amount: 1.00 Lakhs. Duration: 03 days workshop (2016).  PI/Coordinator: Dr. R.K. Garg, Joint Project Director, MPCOST, Bhopal.

Title: Molecular cloning, DNA sequencing and Sequences Analyses” organized from 15th September to 5th October, 2015 for universities and colleges facilities. Funding Agency: DBT, GOI, New Delhi. Sanctioned Amount: 3.25 Lakhs. Duration: 21 days Refresher Course (2015).  PI/Coordinator: Dr. R.K. Garg, Joint Project Director, MPCOST, Bhopal.

Title: Exploration of Molecular Phylogeny and DNA Sequence Analyses for Taxonomy, Biological Diversity and Diseases Parental Association Findings” from 26-28 June, 2014. Funding Agency: DST-SERB, GOI, New Delhi. Sanctioned Amount: 0.75 Lakhs. Duration: 03 days workshop (2014).  PI/Coordinator: Dr. R.K. Garg, Joint Project Director, MPCOST, Bhopal.

Title: Establishment of Human Herbal Health Care Garden (HHHCG). Funding Agency: NMPB, GOI, New Delhi. Sanctioned Amount: 5.00 Lakhs. Duration: Two Years (2009-2011). PI: Dr. Rajesh Sharma.

Title: Molecular Techniques in Species Identification Biodiversity conservation and Phylogenetics” from 10th to 30th January, 2013 for universities and colleges facilities. Funding Agency: DBT, GOI, New Delhi. Sanctioned Amount: 2.50 Lakhs. Duration: 21 days Refresher Course (2013).  PI/Coordinator: Dr. R.K. Garg, Joint Project Director, MPCOST, Bhopal.



Publication and Development


 Sequenced Gene Published/ Deposited in NCBI, USA

 

1.        05 DNA Sequences of MTCOX1 gene, partial CDS of MT-DNA for Puntius ticto (Hamilton, 1822), August,    

          2016|Garg et al. (2016) has been deposited/submitted in NCBI, USA for development of DNA barcodes database.

2.   05 DNA Sequences of MTCYTOB gene, partial CDS of MT-DNA for Catla catla (Hamilton, 1822), 3rdMay 2016|Garg et al. (2016) has been deposited/submitted in NCBI, USA for estimation and analyses of genetic variability and phylogentic positions among the taxonomic groups.

3.        08 DNA gene sequences MTCYTOB gene, partial cds; MT-DNA for Catla catla (Hamilton, 1822), 18thFebruary, 2014|Garg et al. (2014) has been deposited in NCBI, USA for estimation of gene diversity and gene flow among Catla catla (Hamilton, 1822) isolates Fish Federation Pond, Bhopal.

4.        99 DNA gene sequences of Human Mitochondrial Cytochrome b Gene (2014) has been deposited/submitted in NCBI, USA for identification of SNPs responsible for Breast and Ovarian carcinoma. Authors: Chaudhary R, Sairkar M, Sairkar P.K.

5.        04 DNA sequences of internal transcribed spacer (ITS) region of Rauvolfia serpentine (2014) has been deposited/submitted in NCBI, USA for authentication of ITS region as DNA Barcode. Authors: Chouhan S, Sairkar PK, Singh RK

6.        05 DNA sequences of internal transcribed spacer (ITS) region of Cullen corylifolium (2014) has been deposited/submitted in NCBI, USA for authentication of ITS region as DNA Barcode. Authors: Sairkar PK, Chouhan S, Batav N, Singh RK

7.        04 DNA sequences of internal transcribed spacer (ITS) region of Phyllanthus emblica (2014) has been deposited/submitted in NCBI, USA for authentication of ITS region as DNA Barcode. Authors: Sairkar PK, Chouhan S, Batav N, Singh RK

8.        02 DNA sequences of SCAR marker region of Commiphora wightii were submitted. (2013) Authors: Sairkar PK, Sharma A, Shukla NP

9.        03 DNA Sequences for development of species-specific SCAR markers for identification of five medicinal species of Ocimum has been deposited in NCBI, USA FJ948429: gi|227808646|gb|FJ948429.1|[227808646] 2nd May, 2009. P.K. Sairkar, N. Vijay, Garg R.K., N. Silawat, S. Chouhan and N.N. Mehrotra.

 

DNA Barcodes developed and available on www.boldsystems.org,  NCBI, USA

1.        15 DNA barcodes developed using MTCOX1 gene conserved sequences for Tor tor, Puntius ticto, Puntius sarana for Authentication and Identified of un-identified biological samples and available on www.boldsystems.org,  NCBI, USA for identification and phylogentic positioning of the species.

 

Human SNPs (Single nucleotide polymorphism) submitted in ClinVar Database of NCBI, USA

1.        58 Human SNPs of Breast and Ovarian Cancer were Submitted in ClinVar Database of NCBI, National Library of Medicine, National Institute of Health, USA (2014). Out of them 52 were Novel SNPs and used as reference SNPs (refSNPs). Authors: Chaudhary R, Sairkar M, Sairkar PK

 

Primes/Markers Published/ Deposited in Probe databank of NCBI, USA

1.      2 Novel Primers were developed and Submitted in NCBI Probe databank for amplification of complete Human Mitochondrial Cytochrome b gene. (2014) Authors: Chaudhary R, Sairkar M, Sairkar PK

 

2.      4 Novel SCAR Markers were developed and submitted in the NCBI Probe databank for Identification and discrimination of Commiphora species. (2013) Authors: Sairkar PK, Sharma A, Shukla NP

 

List of Research Publication

2017

Sairkar P, Sharma A, Shukla NP (2016) Estimation of Guggulsterone E and Z in the Guggul based commercial formulations using High Performance Thin Layer Chromatography (HPTLC). Journal of Pharmacy and Bioallied Sciences. Article in press.

Sairkar P, Sharma A, Shukla NP (2016) Phenol Free and FTA card based Genomic DNA Extraction from Stems and Leaves of Commiphora wightii and C. myrrha. Acta Physiologiae Plantarum. Under Revision.

2016

Sairkar P, Sharma A, Shukla NP (2016) “SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha,” Molecular Biology International, vol. 2016, Article ID 1482796, 10 pages. doi:10.1155/2016/1482796.

Sairkar P, Sharma A, Shukla NP (2016) “Antimicrobial Activity of Guggulsterone E and Z,” International Journal of Current Microbiology and Applied Sciences, 5(10): 20-26. doi:http://dx.doi.org/10.20546/ijcmas.2016.510.004

2015

Sharma M, Shrotriya A, Chouhan S, Sairkar PK, Singh RK (2015) Diversity assessment of E. coli collected from water bodies of Bhopal using sole source carbon utilization (SSCU) profile. Int. J. Curr. Microbiol. App. Sci (2015) 4(8): 892-906.

2014

Garg R.K., Dhanaxi Chaturvedi, Batav N., R. Sharma and Singh RK (2014) Genetic divergence and gene flow in three populations of Notopterus notopterus (Pallas, 1769), Madhya Pradesh. Journal of Applied Biology & Biotechnology, 2(04), 033-037 (DOI: 10.7324/JABB.2014.2406). 

 Garg R.K. (2014) Intellectual Property Rights and its Role in Biodiversity Conservation at Local and Global Consideration. In: Intellectual Rights and Their Importance in Research, Business and Industry (Eds: R.P. Singh and V.S. Tomar), Published by Daya Publishing House, New Delhi, PP165. 

Garg  R.K., N. Batav and R.K. Singh (2014) Gene flow and estimation of loss of genetic assortments in cultured and wild genotypes of Catla catla (Hamilton, 1822), M.P., India. The Scitech, 1(1): 3-7. 

Garg R.K., P. Sairkar, S. Chouhan, N. Batav, N. Silawat, R. Sharma, R.K. Singh, N.N. Mehrotra (2014) Characterization of genetic variance within and among five populations of Sperata seenghala (Skyes, 1839) revealed by random amplified polymorphic DNA markers. J. Genet. Eng. & Biotech., 12(1): 7-14, http://dx.doi.org/10.1016/j.jgeb.2013.11.001). 

 Garg R.K., N. Batav, N. Silawat and R.K. Singh (2014) Isolation and identification of pathogenic microbes from tomato puree and their delineation of distinctness by molecular techniques. Journal of Applied Biology & Biotechnology, 1(04), 024-031. 

2013

Sairkar P, S Chouhan, N Batav, R Sharma (2013) Optimization of DNA isolation process and enhancement of RAPD PCR for low quality genomic DNA of Terminalia arjuna. Journal of Genetic Engineering and Biotechnology 11 (1):17–24. doi: http://dx.doi.org/10.1016/j.jgeb.2013.02.001.

Sairkar P, N Vijay, N Batav, N Silawat, RK Garg, S Chouhan, R Sharma, NN Mehrotra  (2013) Genetic Variability in Two Economically Important Medicinal Plants of Madhya Pradesh, India and Using RAPD Markers'. Asian J. Exp. Biol. Sci. 4 (1), 36-43.

Silawat N., N. Batav, S. Chouhan, P. Sairkar, R.K. Garg and R.K. Singh (2013) Fungal Flora of Vermicompost and Organic Manure: A case Study of Molecular Diversity of Mucor racemosus using RAPD Analysis. Biosciences Biotechnology Research Asia (2013), 10(2): 509-514. 

Garg R.K., N. Batav and R.K. Singh (2013) Identification of genetic polymorphism in Mystus cavasius (Hamilton-Buchanan, 1822) from Gandhisagar reservoir and Bansagar reservoir, Madhya Pradesh, India. The Journal of Basic and Applied Zoology, 66: 93-100(http://dx.doi.org/10.1016/j.jobaz.2013.06.001). 

Silawat N., N. Batav, S. Chouhan, P. Sairkar, R.K. Garg and R.K. Singh (2013) Fungal Flora of Vermicompost and Organic Manure: A case Study of Molecular Diversity of Mucor racemosus using RAPD Analysis. Biosciences Biotechnology Research Asia (2013), 10(2): 509-514.

2012

Sairkar P, N Vijay, N Silawat, RK Garg, S Chouhan, N Batav, R Sharma (2012) Inter-species Association of Ocimum Genus as Revealed through Random Amplified Polymorphic DNA Fingerprinting. Science Secure Journal of Biotechnology 1 (1), 1-8

Barpete S, M Dhingra, D Parmar, P Sairkar, NC Sharma (2012) Intraspecific Genetic Variation in Eleven Accessions of Grass Pea Using Seed Protein Profile. Science Secure Journal of Biotechnology 1 (1), 20-27

Chandravanshi MK, PK Sairkar, V Sharma, S Chouhan, NP Shukla, (2012) A comparative study of mycoprotein conversion potency of seven different species of Pleurotus from various agro-wastes. International Journal of Agri Science 2 (2), 149-160

2010

Garg R.K., Sairkar P., Silawat N. and Sharma R. (2010) Genetic variability of Mystus cavasius (Hamilton, 1822) populations using Randomly Amplified Polymorphic DNA: National Seminar on Integrated Management of Water Resources with Reference to Biodiversity and Livelihood (Eds.: S.N. Dwivedi, P. Tamot and A.M. Yasim), Academy of Science, Engineering and Technology (ASET), Bhopal. Pp. 90-98.

Garg R.K., Sairkar P., Silawat N., Batav N. and Mehrotra N.N. (2010) Assessment of Genetic Diversity of Clarias batrachus (Linn.) in three water bodies of Bhopal using RAPD markers. Journal of Environmental Biology.

 

Silawat N., Chouhan S., Sairkar P.,Garg R.K.,Vijay N. and Mehrotra N.N. (2010) Estimation of Bacterial Diversity in Soil and Vermicompost Using Sole Source Carbon Utilization (SSCU). African Journal of Microbiology Research, 4 (4): 255-266.

 

2009                                                                                         

Sairkar P., Chandravanshi M. K., Shukla N. P. and Mehrotra N. N. (2009) Mass production of an economically important medicinal plant Stevia rebaudiana using in vitro propagation techniques. Journal of Medicinal Plants Research, 3(4): 266-270.

Vijay N., Sairkar P., Silawat N., Garg R.K. and Mehrotra, N.N. (2009) Genetic variability in Asparagus racemosus (Willd.) from Madhya Pradsh, India by random amplified polymorphic DNA. African Journal of Biotechnology, 8(14): 3135-3140.

Garg R.K., Sairkar P., Silawat N. and Mehrotra N.N. (2009) Discrimination of two Populations of catfish Aorichthys seenghala (Sykes), Madhya Pradesh, India using RAPD fingerprinting. International Journal of Integrative Biology, 7(3):130-134.

Garg R.K., Sairkar P., Silawat N., Vijay N., Batav N. and Mehrotra N.N. (2009) Genetic Diversity between Two Populations of Heteropneustes fossilis (Bloch) Using RAPD Profile. International Journal of Zoological Research, 5(4): 171-177.

Garg R.K.,Silawat N., Sairkar P., Vijay N. and Mehrotra N.N. (2009) RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. African Journal of Biotechnology, 8(17):4032-4038.